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puc19 backbone  (New England Biolabs)


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    Structured Review

    New England Biolabs puc19 backbone
    Puc19 Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc19+backbone/pUC19+Vector/pmc12881585-322-9-17
    Average 97 stars, based on 1404 article reviews
    puc19 backbone - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Compositions comprising a variant polypeptide and uses thereof
    Article Snippet: The variant polypeptides were cloned into a pcDNA3.1 backbone (Invitrogen®). .. The RNA guides (crRNAs) of Table 7 were cloned into a pUC19 backbone (New England Biolabs®). ..

    Article Title: Multifunctional nanoparticle platform for targeted delivery and vaccines
    Article Snippet: .. The expression cassette from the pY71 vector was cloned into the pUC19 backbone (New England Biolabs, Ipswich (MA), cat.# N3041S). ..

    Article Title: Compositions comprising a variant polypeptide and uses thereof
    Article Snippet: Variants of SEQ ID NO: 3 were cloned into a pcda3.1 backbone (Invitrogen®). .. RNA guides were cloned into a pUC19 backbone (New England Biolabs®). ..

    Article Title: The genetic architecture of protein interaction affinity and specificity
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20 μl reactions. ..

    Article Title: The genetic architecture of protein interaction affinity and specificity.
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20μl reactions. ..

    Article Title: Gene editing systems comprising an RNA guide targeting lactate dehydrogenase a (LDHA) and uses thereof
    Article Snippet: The Cas12i2 variants of SEQ ID NO: 1168 and SEQ ID NO: 1171 were individually cloned into a pcda3.1 backbone (Invitrogen). .. Nucleic acids encoding RNA guides E3T1, E3T3, E5T1, E5T9, and E5T10 (Table 7) were cloned into a pUC19 backbone (New England Biolabs). ..

    Expressing:

    Article Title: Multifunctional nanoparticle platform for targeted delivery and vaccines
    Article Snippet: .. The expression cassette from the pY71 vector was cloned into the pUC19 backbone (New England Biolabs, Ipswich (MA), cat.# N3041S). ..

    Polymerase Chain Reaction:

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways.
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences22, designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences , designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Cloning:

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways.
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences22, designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences , designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Synthesized:

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways.
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences22, designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences , designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Plasmid Preparation:

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways.
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences22, designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways
    Article Snippet: .. These five fragments were assembled into the PCR-amplified linearized pUC19 backbone with a primer set using the NEBuilder cloning kit. pReporter–donor–HITI: Sense and antisense strands of the “homology-independent targeted insertion (HITI)” sequences , designed for both the 5′ and 3′ ends, were synthesized, annealed, and inserted into the pReporter–donor vector using an NEBuilder cloning kit. ..

    Article Title: The genetic architecture of protein interaction affinity and specificity
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20 μl reactions. ..

    Article Title: The genetic architecture of protein interaction affinity and specificity.
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20μl reactions. ..

    Binding Assay:

    Article Title: The genetic architecture of protein interaction affinity and specificity
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20 μl reactions. ..

    Article Title: The genetic architecture of protein interaction affinity and specificity.
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20μl reactions. ..

    Sequencing:

    Article Title: The genetic architecture of protein interaction affinity and specificity
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20 μl reactions. ..

    Article Title: The genetic architecture of protein interaction affinity and specificity.
    Article Snippet: .. For the prey intermediate plasmid, a gene fragment was ordered from Twist carrying an SP1 Illumina primer binding site, a 24 bp placeholder barcode sequence (AAGTTCGTTGCATCACCTAGCCAA; prey barcode), a 188 bp random spacer sequence, an SP2 Illumina primer binding site, the CYC promoter, a 3 x Flag-tag fused to a DHFR C-terminal half (from here on referred to as FR-tag), a Linker sequence (Linker L4, GASGSAAGGSGSAGSGASAS), the JUN bZIP wt sequence (consisting of the wt DNA-binding domain (DBD) and the five heptads of the wt zipper domain), and Linker L3 was cloned into the pUC19 backbone which had been digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and EcoRI (New England Biolabs, Ipswitch, MA) via Gibson assembly using 62.2 fmol of backbone and 121.4 fmol of gene fragment in 20μl reactions. ..



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